Insertion junction: CLIP2.006443_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.006443
Chromosome:chromosome 12
Location:6032001
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g534650 (1 of 66) 2.7.12.1 - Dual-specificity kinase 5'UTR
Cre12.g534700 FKB19 (1 of 1) PTHR10516:SF179 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP19, CHLOROPLASTIC; peptidyl-prolyl cis-trans isomerase, FKBP-type outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAAATTCGCCGGGTGGAATGATCGGGCACACGTCAGTCCAAAAAGGGCC
Internal bar code:GGCTTACCCTTGTTGGTTTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:715
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GCCACAGTGGTAACTTCGGA
Suggested primer 2:GGAGGTTGCATCGCGAATTC