| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.006443 |
| Chromosome: | chromosome 12 |
| Location: | 6032005 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534650 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | 5'UTR | |
| Cre12.g534700 | FKB19 | (1 of 1) PTHR10516:SF179 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP19, CHLOROPLASTIC; peptidyl-prolyl cis-trans isomerase, FKBP-type | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACCCCCGCTTCTTCGTCGCTATTGCAACGCGCTAGGCCTACAAGTTTT |
| Internal bar code: | GGCTTACCCTTGTTGGTTTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2999 |
| LEAP-Seq percent confirming: | 57.6923 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGTTGCATCGCGAATTC |
| Suggested primer 2: | GCCACAGTGGTAACTTCGGA |