Insertion junction: CLIP2.006445_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.006445
Chromosome:chromosome 9
Location:1902113
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre09.g397400 SDR17 Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.300//1.3.1.33 - NADP-retinol dehydrogenase / Retinol dehydrogenase (NADP(+)) // Protochlorophyllide reductase / Protochlorophyllide oxidoreductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCGTACGCAAATATTCCGCGCCTAACTTGTCCGCCCGTCCTGTGGCGC
Internal bar code:ATCTTATTCGACGGTCTGGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:177
LEAP-Seq percent confirming:55.5556
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TCGGCCACTTCCTCCTCATA
Suggested primer 2:CGAAGGAAGATACGAGCGCT