| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.006514 |
| Chromosome: | chromosome 12 |
| Location: | 1268164 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489700 | OTC1 | Ornithine carbamoyltransferase; (1 of 1) 2.1.3.3 - Ornithine carbamoyltransferase / OTCase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCTGATGCCTCCGGGCGGCAGGGCCAAGGCCGCTGGGGACCGAGCGC |
| Internal bar code: | TACTCCTAACCGTAAAATCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2290 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGGAGCGTGGGTATGTG |
| Suggested primer 2: | TATGATTGTCCGCCCATCCG |