| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.006636 |
| Chromosome: | chromosome 3 |
| Location: | 6858319 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197650 | (1 of 17) PF04755 - PAP_fibrillin (PAP_fibrillin) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCGCCGGCTGCATGGTGCCGGCACCTACATGGCATCGCAAGGCGTCG |
| Internal bar code: | AAGCGTTGGCTCGACGATTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1135 |
| LEAP-Seq percent confirming: | 28.125 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGCTGGGAACTTCTACTT |
| Suggested primer 2: | GCACGATACAGAGCCCTACC |