| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.006648 |
| Chromosome: | chromosome 12 |
| Location: | 3100495 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500950 | CLPP4 | (1 of 3) PTHR10381//PTHR10381:SF24 - ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT // SUBFAMILY NOT NAMED; active subunit of the chloroplast ClpP complex | 3'UTR |
| Cre12.g501000 | PPT1,TPT22,TPT20 | Putative UDP-GlcA transporter; (1 of 4) PTHR11132//PTHR11132:SF97 - SOLUTE CARRIER FAMILY 35 // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTAGTCGGGGGGCTATTGACGAGTATGACGATGTTCATTTACAGCGGT |
| Internal bar code: | ATTAGTTCCCTTATTTCCAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2635 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAATTGGAAGGCATGGCA |
| Suggested primer 2: | GTCTCCTGGTCCTAGGCTGA |