Insertion junction: CLIP2.006682_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.006682
Chromosome:chromosome 3
Location:2153341
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g156900 LHCBM5 (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chlorophyll a/b binding protein of LHCII intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGCACATTCGACCTGGGCCTCGCAACTACGCTTCCACGCACCGGATGG
Internal bar code:AATGTGAGTAATAAAAGTACCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2330
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCATGCGCTAGGAGATGC
Suggested primer 2:GCAAATCACGCACCGTTCTT