| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.006772 |
| Chromosome: | chromosome 17 |
| Location: | 1747648 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708700 | HTR20,HTR27 | Histone H3; (1 of 35) K11253 - histone H3 (H3) | gene_edge/mRNA_edge/5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTTCAAATTTCTTCTGTAAACTTCCAACAAAATGGCCCGCACCAAGCA |
| Internal bar code: | TCTGATAAATCATGTACGTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2027 |
| LEAP-Seq percent confirming: | 89.4737 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCCATCCCAACCCATTAA |
| Suggested primer 2: | CGGATAGCGGGCTTGGTAAT |