Insertion junction: CLIP2.006824_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.006824
Chromosome:chromosome 17
Location:4505861
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g731350 SDR28 Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.184//1.1.1.189//1.1.1.197 - Carbonyl reductase (NADPH) / Xenobiotic ketone reductase // Prostaglandin-E(2) 9-reductase / Prostaglandin-E2 9-reductase // 15-hydroxyprostaglandin dehydrogenase (NADP(+)) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCAGCGCCGCCTACAGGGGCGTCTGACCTAGCCCGAAGCCCCGAATAC
Internal bar code:GATCCGCCCCACGGGAACATCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1159
LEAP-Seq percent confirming:47.619
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGACGTGTGTTGTGTTTT
Suggested primer 2:GTACTCTCCCAATCGAGCCG