Insertion junction: CLIP2.006894_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.006894
Chromosome:chromosome 16
Location:2663162
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g661550 (1 of 1) PF06941 - 5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C) (NT5C) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTAAATCTTAGACGACGGCGTGCTCAGCAGTCAGCACACATTTACATCA
Internal bar code:GGGTTTCGATCTCGGTAACGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2030
LEAP-Seq percent confirming:95.2381
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:GCACGGCGAAACGACATTTA
Suggested primer 2:ACACCTTGCCATGCTATGCT