| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.006927 |
| Chromosome: | chromosome 4 |
| Location: | 157132 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g216850 | TUA2 | (1 of 2) K07374 - tubulin alpha (TUBA); Alpha tubulin 2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCTTACTCCCAACAACACTCCACAAACACAAGTACCGTTGGTACGCAG |
| Internal bar code: | ACTAGGCTTAAAAAATTCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4170 |
| LEAP-Seq percent confirming: | 94.2857 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAATAGCAAGCGAGAAGCC |
| Suggested primer 2: | GATTACGCTCACGCAAGCTG |