| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.006992 |
| Chromosome: | chromosome 12 |
| Location: | 4407854 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g519900 | (1 of 1) 6.3.2.13 - UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase / UDP-N-acetylmuramyl-tripeptide synthetase | 5'UTR | |
| Cre12.g519950 | FAP42,GUK3,C1b-350 | Flagellar Associated Protein 42; (1 of 1) PTHR23359//PTHR23359:SF92 - NUCLEOTIDE KINASE // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAATTGCGCGTTTCTGCGAAGGCTCCTTGTTTGCACACATATCAAGCAT |
| Internal bar code: | CGCGGACAATCGTGACCAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1202 |
| LEAP-Seq percent confirming: | 76.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCGGAAGACTTCACTTGC |
| Suggested primer 2: | TGACGCCTTACCAGCTTCAG |