Insertion junction: CLIP2.007095_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.007095
Chromosome:chromosome 12
Location:8615315
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g548400 LHCBM2 (1 of 3) K08913 - light-harvesting complex II chlorophyll a/b binding protein 2 (LHCB2); Light-harvesting protein of photosystem II CDS/5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATATCAAAATGGCCGCCATCATGAAGTCCGCTGTTCGCAGCTCCGTGC
Internal bar code:ATTGTCATGTACTTGGCGGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2078
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TGGATCAGGTTCTCGTTGCC
Suggested primer 2:ACTCGTTCTACGCGCCTATG