| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.007129 |
| Chromosome: | chromosome 13 |
| Location: | 2301312 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578750 | TBA1 | (1 of 1) K00207 - dihydropyrimidine dehydrogenase (NADP+) (DPYD); Translation factor for chloroplast psbA mRNA | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAAGGGGTCCTGGTAGAGGACGGGCCGATGGGATGTCGGCAGCGTGGT |
| Internal bar code: | GGAATAAGTTTTACCTTATTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 393 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTCTTTCTCGCACTCAA |
| Suggested primer 2: | CAGCCAGCATCACGTCAAAG |