Insertion junction: CLIP2.007254_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.007254
Chromosome:chromosome 8
Location:242843
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre08.g358562 CPN20 Chaperonin 20; (1 of 2) PTHR10772//PTHR10772:SF0 - 10 KDA HEAT SHOCK PROTEIN // 20 KDA CHAPERONIN, CHLOROPLASTIC 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAGCGCCTGCGCCGCCGCGCACGGCTCCCGCCGTGACCCACTCACCAGC
Internal bar code:GCAATGTCATGCCTTGGGTGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:572
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GCCACCGTCATGTACTCCAA
Suggested primer 2:GACCCCTTGACCCTTGGATG