Gene: Cre08.g358562
Overview
| Common name: | CPN20 |
| Defline: | Chaperonin 20; (1 of 2) PTHR10772//PTHR10772:SF0 - 10 KDA HEAT SHOCK PROTEIN // 20 KDA CHAPERONIN, CHLOROPLASTIC |
| Description: | Co-chaperonin assisting the CPN60 chaperonin in protein folding; homologous to bacterial GroES; contains two cpn10 domains; located in the chloroplast, where one Cpn23 form with Cpn10 and two Cpn20 a tetramer with seven cpn10 domains; heptamers of Cpn10-like co-chaperonins bind to one of the two Cpn60 heptameric rings and trigger ATP hydrolysis; also found in association with the ClpPR complex |
| Synonyms: | CPN20,Cre28.g776900.t1.2,g8378.t1,Cre28.g776900.t1.1 |
| Chromosome: | chromosome 8 |
Protein Localization
A plasmid for expressing Cre08.g358562 tagged with the fluorescent protein Venus and affinity epitope 3xFLAG is available: pLM005-Cre08.g358562-Venus-3xFLAG
Imaged strain: CSI_LW11B3
Imaging conditions: Tris-Phosphate media, air, 150 uM photons/m2/s, shaking in 96-well plate; imaged after 16 hours.
Classification(s):
chloroplast/not homogeneous/not pyrenoid depleted/enriched in stroma and around pyrenoid
shapes in cytoplasm/uncategorized shapes in cytoplasm
If you reference these protein localization data in a manuscript, please cite: Wang et al. 2023.
 |  |  |  |
| Venus | Chlorophyll | Bright field | Merged |
Protein-Protein Interactions
We did not determine any protein-protein interactions for this gene.
Mutant Strains
| Insertion Junction | Mutant Strain | Insertion Junction Feature | Confidence (%) | Genome Version |
|---|---|---|---|---|
| CLIP2.003535_1 | CLIP2.003535 | 3'UTR | 80 | 6.1 |
| CLIP2.003810_1 | CLIP2.003810 | intron | 80 | 6.1 |
| CLIP2.003810_2 | CLIP2.003810 | intron | 80 | 6.1 |
| CLIP2.005300_1 | CLIP2.005300 | 3'UTR | 63 | 6.1 |
| CLIP2.007254_2 | CLIP2.007254 | 3'UTR | 63 | 6.1 |
| CLIP2.007586_1 | CLIP2.007586 | intron | 80 | 6.1 |
| CLIP2.007586_2 | CLIP2.007586 | intron | 80 | 6.1 |
| LMJ.RY0402.102214_3 | LMJ.RY0402.102214 | 3'UTR | 73 | 5.5 |
| LMJ.RY0402.117920_1 | LMJ.RY0402.117920 | 3'UTR | 95 | 5.5 |
| LMJ.RY0402.117920_2 | LMJ.RY0402.117920 | 3'UTR | 95 | 5.5 |
Gene Annotations
| PFAM: | PF00166 |
| PANTHER: | PTHR10772 PTHR10772:SF0 |
| KEGG_ec: | |
| KEGG_orthology: | K04078 |
| KOG: | KOG1641 |
| Gene ontology terms:: | GO:0005737, GO:0006457 |
| Best arabidopsis TAIR name: | AT5G20720 |
| Best arabidopsis TAIR symbol: | ATCPN21,CHCPN10,CPN10,CPN20,CPN21 |
| Best arabidopsis TAIR defline: | chaperonin 20 |
| Phytozome Locus Page |
Phenotypes
Our phenotype screens did not find statistically significant results for this gene.