Insertion junction: CLIP2.007586_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.007586
Chromosome:chromosome 8
Location:243179
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g358562 CPN20 Chaperonin 20; (1 of 2) PTHR10772//PTHR10772:SF0 - 10 KDA HEAT SHOCK PROTEIN // 20 KDA CHAPERONIN, CHLOROPLASTIC intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGTACGCAACTGTAGACGGCCGCTCACCACACGTGTGTTCCTGTGTGT
Internal bar code:ACGACATCAGTCTACAGCGTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1554
LEAP-Seq percent confirming:95.3488
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCGACTCCTGTTGTCACA
Suggested primer 2:CACACACGCACACTCTCTCT