| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.117920 |
| Chromosome: | chromosome 8 |
| Location: | 282211 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358562 | CPN20 | Chaperonin 20; (1 of 2) PTHR10772//PTHR10772:SF0 - 10 KDA HEAT SHOCK PROTEIN // 20 KDA CHAPERONIN, CHLOROPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTGCTGCCATCCAGTCGGCAGGTGCCCG |
| Internal bar code: | AGCTTGGCGGGGCCCATGCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 780 |
| LEAP-Seq percent confirming: | 98.816 |
| LEAP-Seq n confirming: | 2170 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGAGTTCGAGGTGAGGC |
| Suggested primer 2: | AGTACAGCGAGCTAGGCAGC |