Insertion junction: CLIP2.007260_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.007260
Chromosome:chromosome 12
Location:8614425
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g548400 LHCBM2 (1 of 3) K08913 - light-harvesting complex II chlorophyll a/b binding protein 2 (LHCB2); Light-harvesting protein of photosystem II CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACTGGGCGTGGATCAGGTTCTCGTTGCCGAGGTAGTTCAGGCCGCCCTC
Internal bar code:TGACGGGCGACGTGCGCGCTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:3387
LEAP-Seq percent confirming:71.4286
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CCACTTGCCTCTCAGCTGAA
Suggested primer 2:GCCAGGCACTCCACTAACAT