Insertion junction: CLIP2.007314_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.007314
Chromosome:chromosome 6
Location:5385120
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g285401 HLP1,FAP355 Histone-like Flagellar Associated protein; (1 of 1) PF00216 - Bacterial DNA-binding protein (Bac_DNA_binding) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCATTTGGCCCATTCCGCCTGTAAAGTTGGGCTGGGTAATGGGAATGT
Internal bar code:CTTGTTCGTTGCGACCACTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:780
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGACTGCTGGCCACATTACT
Suggested primer 2:CACCTTTCGTGACTCAGCCT