| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007368 |
| Chromosome: | chromosome 17 |
| Location: | 421115 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698750 | RAA12,OPR104 | psaA mRNA trans-splicing factor of subcomplex I; (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGCCGCGCGCGGACCCCGTCGCCCAACTAGTTACAGATCTATACTAAC |
| Internal bar code: | TGCGGCTCCCCCCGACCCGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 158 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCATCAACTCCACAAGCT |
| Suggested primer 2: | GCCGCTATCACTCTTTCCGA |