Insertion junction: CLIP2.007376_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.007376
Chromosome:chromosome 12
Location:8613531
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g548400 LHCBM2 (1 of 3) K08913 - light-harvesting complex II chlorophyll a/b binding protein 2 (LHCB2); Light-harvesting protein of photosystem II 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTCCGGAAAAACAATTACCGCTGCGCGACGCAACAAACCACCAGCTCAC
Internal bar code:CACTGAACATTCAAGGATTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:4290
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:TCGCTGAGCTGAAGGTGAAG
Suggested primer 2:TGTGGTGCTTGCGTTTGATG