Insertion cassette: | CIB2 |
Side of cassette: | 5' truncated? |
Strand: | - |
Strain: | CLIP2.007413 |
Chromosome: | chromosome 2 |
Location: | 6797072 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g388060 | VDR1 | violaxanthin de-epoxidase related, chloroplast; (1 of 1) 1.10.99.3 - Transferred entry: 1.23.5.1 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCAGAGAACAACTATATGGCGTCCTGTGTGTGCGTGTGTGGGGCCTGG |
Internal bar code: | AACGGTGGATGCTTGAAGTCAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1196 |
LEAP-Seq percent confirming: | 97.6744 |
LEAP-Seq n confirming: | 42 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGCCACTAGCTACTCCCCA |
Suggested primer 2: | TGGTGGTCTTATGACACGCC |