Insertion junction: CLIP2.007451_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.007451
Chromosome:chromosome 16
Location:3629427
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g674534 (1 of 1) IPR012336//IPR013026//IPR013105//IPR019734 - Thioredoxin-like fold // Tetratricopeptide repeat-containing domain // Tetratricopeptide repeat 2 // Tetratricopeptide repeat 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAATTGACCTAGAGTAGATTGCACGTGTAACACTATCATGCAAAGCGGTC
Internal bar code:TCGTCGGGCCAGGGGGTTGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:5245
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCTTGACCTCGGAGATAC
Suggested primer 2:GGATGACAGTGAGGTAGGCG