| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.007567 |
| Chromosome: | chromosome 2 |
| Location: | 7091437 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390060 | RAA4 | psaA mRNA trans-splicing factor | CDS |
| Cre09.g390097 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGACAGAGCGGCTGGTGGCGCCGGAGAGGTCTTCGGATGCGGAGGCA |
| Internal bar code: | AAAAGCATACGGCATACGTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3482 |
| LEAP-Seq percent confirming: | 92.7419 |
| LEAP-Seq n confirming: | 115 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 124 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGCCCAACAAATGTTCA |
| Suggested primer 2: | CCTTTCTTCTCGCTGGAGGC |