Insertion junction: CLIP2.007579_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.007579
Chromosome:chromosome 1
Location:6066487
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g042800 DVR1 3%252C8-Divinyl protochlorophyllide a 8-vinyl reductase, chloroplast precursor; (1 of 1) K19073 - divinyl chlorophyllide a 8-vinyl-reductase (DVR) 5'UTR
Cre01.g042850 (1 of 2) K17278 - membrane-associated progesterone receptor component (PGRMC1_2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATGGACAATTGACTCGTGGCGCGGGAATCCCAGTAGGCAACTCTAAGG
Internal bar code:TGGTATTGACTTACGGTAGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:346
LEAP-Seq percent confirming:12.5
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTTGCGAGCAAGATAGC
Suggested primer 2:TCTTCCGATTGTGCCTCCAC