Insertion junction: CLIP2.007613_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.007613
Chromosome:chromosome 3
Location:2155640
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g156900 LHCBM5 (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chlorophyll a/b binding protein of LHCII 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCGTTCGGATTGGATAAGGGAGGCTGTGACTGAGGATTCATGCTTCCC
Internal bar code:CTCCGATGGTAAATCTAACCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:4430
LEAP-Seq percent confirming:98.9691
LEAP-Seq n confirming:96
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:97

Suggested primers for confirmation by PCR

Suggested primer 1:CGTACATACCGTGGACCGAG
Suggested primer 2:TCCTGCGCTTCTTCAAGGAG