Insertion junction: CLIP2.007618_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.007618
Chromosome:chromosome 1
Location:6063504
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g042800 DVR1 3%252C8-Divinyl protochlorophyllide a 8-vinyl reductase, chloroplast precursor; (1 of 1) K19073 - divinyl chlorophyllide a 8-vinyl-reductase (DVR) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATGTGACAGCTGGAGGACAGCGCCGAATTCGCGCGCATTGGCAAGTAC
Internal bar code:CTTTTTAGCACTCCATATCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:4403
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGTAATCCCCTACTTCCC
Suggested primer 2:TCACCGAGCAGAACAAGGTC