Insertion junction: CLIP2.007795_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.007795
Chromosome:chromosome 1
Location:6680755
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g047600 SDR4 Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.121 - Aldose 1-dehydrogenase (NAD(+)) intron
Cre01.g047650 (1 of 1) K10268 - F-box and leucine-rich repeat protein 2/20 (FBXL2_20) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTTTCGGTGCCAGAGCTAACCCTGTGTAAATTTCTTTGCGGGTATGGG
Internal bar code:GCCGCTTAGAGTGTCTTGCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2402
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:TACCTTCAACCAGCGACGAC
Suggested primer 2:AACCGTAGCCGTGGGTTTAG