| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.007965 |
| Chromosome: | chromosome 12 |
| Location: | 5935977 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533750 | (1 of 11) PF00632 - HECT-domain (ubiquitin-transferase) (HECT) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCGGAAACTTCAACCCTGGTTTCATCTTTGCCGGCCTAGTCGCTGGG |
| Internal bar code: | GGCGATCGGCCGTAGTCTTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1502 |
| LEAP-Seq percent confirming: | 78.9474 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCACTATCTGGCCGAGACG |
| Suggested primer 2: | TGACGGATAATAGGCGGCAC |