| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.008069 |
| Chromosome: | chromosome 2 |
| Location: | 1804144 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g086850 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATTGGCATTGGCAGCGGCGATGGTATCAGGGTGGCTCGAGACCATTGG |
| Internal bar code: | GAGTCGCGGTTCAGCATCTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1817 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCCAGTCTGGATGAAGGC |
| Suggested primer 2: | TTATTGGTGGGCGTCTGCAT |