| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.008570 |
| Chromosome: | chromosome 10 |
| Location: | 6128236 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462250 | REF1 | RNA Export Factor; (1 of 1) K12881 - THO complex subunit 4 (THOC4, ALY) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCCCCTCCTCCTCCGCATGCCCCGCCCCGCCCACTACACCCACGCCT |
| Internal bar code: | CTTTCCACTGCGAGTGAGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 402 |
| LEAP-Seq percent confirming: | 21.4286 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCGGCTGACCTGTCACTG |
| Suggested primer 2: | CAGCTAGCGATGTTAGCCGA |