| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.008940 |
| Chromosome: | chromosome 17 |
| Location: | 2947402 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g719500 | AOF9 | (1 of 2) 1.3.99.29 - Phytoene desaturase (zeta-carotene-forming) / 2-step phytoene desaturase; Flavin-containing amine oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCCCGACTCCCGTTCACGAGCCAGATTGTGCGGTCACCGTAAATGACC |
| Internal bar code: | CTGTGTGTCGTACGTGGTCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2587 |
| LEAP-Seq percent confirming: | 92.5 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGCCGTGCAACATCAAA |
| Suggested primer 2: | TCTAACCACACCTGCTGCTG |