| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.009466 |
| Chromosome: | chromosome 3 |
| Location: | 6501457 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193800 | TSN1 | Aspartyl-tRNA synthetase; (1 of 1) 6.1.1.22 - Asparagine--tRNA ligase / Asparaginyl-tRNA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTATCTTGGTGGCTGTTCCACAGGTGACCACGCTGCTGGGCAAGATGA |
| Internal bar code: | ATTGGGCCCTGGGTTTATCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1430 |
| LEAP-Seq percent confirming: | 73.8095 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGCTGCATAATTCCCGC |
| Suggested primer 2: | TGAGCTGAAGGCAGTCTGTG |