| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.009998 |
| Chromosome: | chromosome 4 |
| Location: | 186196 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217000 | intron | ||
| lncRNA_TCONS_00237454 | intron | ||
| lncRNA_TCONS_00237455 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTTCTAGGGTTCAAACATGCGCTAAGAAGCTCCGATAGCTTGCGCCCA |
| Internal bar code: | TCATTACCTTAGACTGTGTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1653 |
| LEAP-Seq percent confirming: | 50.6944 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 71 |
| LEAP-Seq n unique pos: | 144 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCGTACCGTATCTGGAC |
| Suggested primer 2: | CTTTTCCCACAGGCACATGC |