Insertion junction: CLIP2.010079_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.010079
Chromosome:chromosome 17
Location:4179061
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g729250 UPF2 (1 of 1) K14327 - regulator of nonsense transcripts 2 (UPF2, RENT2); Putative UPF2 regulator of nonsense transcripts intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCTGGTTGATGCTTGGTGCTTAGGCTGGTTTGGCCTATGCTGAATGAT
Internal bar code:TTAGTGTGGTATCAGTGACACT

Confirmation - LEAP-Seq

LEAP-Seq distance:585
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCACTTGACCACGTACTG
Suggested primer 2:ACTCAAGAAGTGAAGGCGGG