Insertion junction: CLIP2.010159_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.010159
Chromosome:chromosome 11
Location:2426420
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095121 PUS9 (1 of 1) PTHR21600//PTHR21600:SF11 - RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE B // PSEUDOURIDINE SYNTHASE FAMILY PROTEIN; RNA pseudouridine synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTCCATCTCTAACACCCGCGCTCACGCAGGGCGCTCACGCCGGCAGAC
Internal bar code:TTGAGCGAGTTTTTATTCAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1866
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:108
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:108

Suggested primers for confirmation by PCR

Suggested primer 1:AGCAGATACCGCAGCTGATC
Suggested primer 2:TACAAACTCCCTCACGTCGC