| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.010170 |
| Chromosome: | chromosome 3 |
| Location: | 5132799 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g180800 | HDA16 | Histone deacetylase complex protein; (1 of 1) K11644 - paired amphipathic helix protein Sin3a (SIN3A) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGAATTCGCCCACCTTGCTGGTGGTGCAGCTGATCTTGCACTCCAGGC |
| Internal bar code: | TCAATTGTGCCCTCGTTCGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3349 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCACCTTGTACCACGTCA |
| Suggested primer 2: | GTCACAACAACACGCAGGTC |