Insertion junction: CLIP2.010469_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.010469
Chromosome:plastome
Location:63878
Confidence (%):80
Locus systematic id Locus common name Defline Feature
CreCp.g802291 ChreCp027,psbZ,2716976 photosystem II reaction center Z protein; (1 of 1) K02724 - photosystem II PsbZ protein (psbZ) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGAGTTGCAAAAACTACAGGTACACCTACTACTAAAGCGAAAGAAACAA
Internal bar code:TAGCCAAGGTATAGGGCAATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:143
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCCACTGACGTCCACTAA
Suggested primer 2:CGCAACTGCCTCCTTCGTAA