| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.010592 |
| Chromosome: | chromosome 12 |
| Location: | 1202779 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490300 | MSD4 | (1 of 5) K04564 - superoxide dismutase, Fe-Mn family (SOD2); Mn superoxide dismutase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGGGGAGGCTGAACTGCATTGGCCAGGGGAATGTGTTGCTGGCGTCC |
| Internal bar code: | TCGTTGATCTAATAGTATAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1710 |
| LEAP-Seq percent confirming: | 88.5714 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTGGTCCAGCGTACGTGT |
| Suggested primer 2: | CCTACACCCACGTACACACC |