| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.010921 |
| Chromosome: | chromosome 2 |
| Location: | 824043 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g078750 | PUS3 | RNA pseudouridine synthase; (1 of 1) K15452 - tRNA pseudouridine synthase 2 [EC:5.4.99.-] (PUS2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTAACTTTAACCGCGTGTGTGTCTGTGTGTGTGTAAGTTCTTCGGTGC |
| Internal bar code: | GGTTGTGGAAGTTATCGAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 253 |
| LEAP-Seq percent confirming: | 10.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTCTTGTGAGTGCCGTCT |
| Suggested primer 2: | AAAGGAGCTCCCGTCCCTAT |