Insertion junction: CLIP2.010932_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.010932
Chromosome:chromosome 1
Location:6679830
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g047600 SDR4 Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.121 - Aldose 1-dehydrogenase (NAD(+)) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTCCAGCACACGGGCGCTCCAGTCCGAGCCAGGCTGCGAGGTGCGGCG
Internal bar code:AAGTGGGCTGAGGTTAAACTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1446
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTGTTGGACGTTGACG
Suggested primer 2:TGCTGTCCAAAGGCTATCGG