Insertion junction: CLIP2.011357_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.011357
Chromosome:chromosome 13
Location:1620307
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g573450 CPS Putative cleavage and polyadenylation specificity factor subunit 1; (1 of 1) K14401 - cleavage and polyadenylation specificity factor subunit 1 (CPSF1, CFT1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCACTAAACCCCCACCTCGAGGCCACGTGGCCACCGCCCCCCCCCCCTG
Internal bar code:TTTACTGAAGAATACGACGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:777
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGAAGGAGGAGCAAGGCG
Suggested primer 2:TTCCCAAGTGGCAGCAGTAG