Insertion junction: CLIP2.011545_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.011545
Chromosome:chromosome 5
Location:1362832
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g234600 (1 of 100) IPR001810 - F-box domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGAAGTTGTCAGGAACGGGAAGTACCCGCGTTACATTCTCCCCCCCTTG
Internal bar code:ATGAGGCGACTGCCGACGGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1227
LEAP-Seq percent confirming:57.8947
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CGAAGGAACATGTTAGCGCG
Suggested primer 2:CACACCCTTCCGTCTTCACA