Insertion junction: CLIP2.011957_4

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.011957
Chromosome:chromosome 12
Location:9319916
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g542100 (1 of 2) 3.4.21.26 - Prolyl oligopeptidase / Prolyl endopeptidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAGGTCGGGTCGCTGGTTGGCGCACGCCGCCACAAGCAGGCCGCCGTTA
Internal bar code:CTACGGGTTTCGACTAATCAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1268
LEAP-Seq percent confirming:47.0588
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGTGCTGCTTGTCAGATA
Suggested primer 2:TTTGGCGTTCATGCACTTGG