| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.012572 |
| Chromosome: | chromosome 12 |
| Location: | 5410134 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g529350 | SMM42 | (1 of 1) 2.1.1.163//2.1.1.201 - Demethylmenaquinone methyltransferase / Demethylmenaquinone C-methylase // 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase; S-adenosyl-L-methionine-dependent methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTACTGCTCGTGCTGGCTGCCGGCGCTGGCTCGTCCTCCCGTGGGGGC |
| Internal bar code: | TAGCGGCACTTATTAAGATAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 628 |
| LEAP-Seq percent confirming: | 37.5 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCTTCGCAGTGTCTGGT |
| Suggested primer 2: | GGGGCAGCAAGAGTGTTACT |