| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.012700 |
| Chromosome: | chromosome 16 |
| Location: | 7526120 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682699 | 3'UTR_intron | ||
| Cre16.g682811 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAGGCGCTGCTTCGCAACCACACCCTCAACCCGCTGCTGGGCGGCATC |
| Internal bar code: | CGCGATGCATGATTAAACTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2411 |
| LEAP-Seq percent confirming: | 23.5294 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACACAAGGAACGTGCAAG |
| Suggested primer 2: | CCTACGGTAGCGTTGGATCC |