Insertion junction: CLIP2.013360_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.013360
Chromosome:chromosome 14
Location:1144988
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g614950 MRPS2,uS2m (1 of 1) PF00318//PF01391 - Ribosomal protein S2 (Ribosomal_S2) // Collagen triple helix repeat (20 copies) (Collagen); Mitochondrial ribosomal protein S2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGAATCCAAGTGCTGCGCCCTGCGAAACCCTTCATCCCACCTGCAGGCG
Internal bar code:CAGTTCACAGGACGGCAGACCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1690
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCACACACCTACATGGCT
Suggested primer 2:ATGGGCTTTGGAGGTACAGC