Insertion junction: CLIP2.013396_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.013396
Chromosome:chromosome 3
Location:6755279
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g196450 (1 of 1) K15178 - RNA polymerase-associated protein RTF1 (RTF1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACTGCCACATGCTCAAGTCTTGTGGAAGTTGCGCGTGCCAGCGGCACCC
Internal bar code:CTGTTGCGTTGTACACTATGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3148
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:TCTGCCTCCTTAGCCTTTGC
Suggested primer 2:CTGACGACCTAGACGATGCC