| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.013612 |
| Chromosome: | chromosome 3 |
| Location: | 6500413 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193800 | TSN1 | Aspartyl-tRNA synthetase; (1 of 1) 6.1.1.22 - Asparagine--tRNA ligase / Asparaginyl-tRNA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCACACGCACACACCTGCAGCTGGCCGGACACGGTGAGGAAGGCGGG |
| Internal bar code: | TTAAGGAAAGCCCCGATACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2303 |
| LEAP-Seq percent confirming: | 45.0704 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGGAGGACAAGTCGCTG |
| Suggested primer 2: | ATGACGTATAGGTGCGGCTG |